A new, simple and rapid spectrophotometric method was developed for determination of Acebutolol Hydrochloride (ACE) in pure and pharmaceutical formulations. The solvent and wavelength of detection were optimized in order to maximize the sensitivity of the proposed method. Parameters such as linearity, precision, accuracy, limit of detection, limit of quantification and content uniformity were studied according to the International Conference on Harmonization (ICH) Guidelines. The linearity was established between the concentration ranges of 2 – 12 µg/ml. The intra- and inter-day relative standard deviation (RSD) was less than 2 %. Limits of detection and quantification were determined as 0.23 and 0.69 µg/ml, respectively. The mean recovery value of Acebutolol HCl was 99.50% for pharmaceutical preparation. The method was applied for the quality control of commercial Acebutolol HCl dosage form to quantify the drug and to check the formulation content uniformity.
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